The biuret reagent free essay sample

In this practical exercise you will be using biuret reagent. Why is biuret reagent used in this practical? The biuret reagent is used to assess the concentration of the protein because peptide bonds occur with the same frequency per amino acid in the peptide. The intensity of the colour, and therefore the absorption, is directly proportional to the protein concentration. Q 2. What is the experimental reason for constructing a standard curve? Multiple samples with known properties can be measured and graphed, which then enables the same properties to be determined for the unknown samples, in this case the concentration, by interpolating the graph which depicts the relationship between the absorbance and concentration. Q 3. Based on the above information (in the prac manual) and your own thinking, which plant do you hypothesize will have more rubisco, one raised in the sun or one raised in shade? It is hypothesised that the sun raised silverbeet plant will have more rubisco than the shaded silverbeet plant. We will write a custom essay sample on The biuret reagent or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page Should the line connect all the data points or should it be a line of best fit? Why? (2 sentences) A line of best fit as it will give an accurate representation of the relationship between absorbance and concentration between the different samples. The more samples used, the closer the results would be to the line of best fit. Q 5. Is it possible to determine the protein concentration of a solution from the standard curve if the measured absorbance is off the scale of the y-axis, i. e. higher than the highest y-value on the standard curve? Give your reasons. 4 sentences) It’s not possible to determine the protein concentration because the data gathered from the standard solutions is in a certain range (specific scale). Therefore the relationship between absorbance and concentration cannot be determined for anything outside of this range. Q 6. Describe  two  ways in which you would modify the experimental procedure to determine the protein concentration of this solution? Gi ve full details of the two experimental procedures. (2 and 4 sentences) One way in which the experimental procedure could be modified is diluting the sample. This involves decreasing the volume of BSA Protein solution and increasing the buffer volume by the same amount. As a result the absorbance would be lower and fit on the graph. Another way may be to extend the curve by increasing the concentration of the BSA Protein solution which would provide a greater range. Do your results support your hypothesis regarding these tissue samples? (2-3 sentences) The hypothesis regarding the tissue samples was supported. The absorbance of the sun raised silverbeet plant was found to be 0. 801, which was greater than the shaded silverbeet plant with an absorbance of 0. 69. Q 8. One possible reason for the results not supporting your experimental hypothesis is experimental error. List three examples of possible sources of error that may have occurred in your work in this practical class. (2-3 sentences) A possible error may be adding inaccurate amounts of buffer between samples, causing them to dilute either excessively or insufficiently, resulting in an inaccurate standard curve. Another error could be leaving the sample solutions for over 25 minutes, which could cause the protein to start degrading. Not zeroing the spectrophotometer or cleaning the tube properly can affect the absorbance value. Q 9. Even if your results do support the experimental hypothesis, give three major possible explanations for a  negative result  (i. e. biological reasons not experimental errors). (4-5 sentences) Biological errors involve using different parts the plant, such as a stem or leaf and one may have a higher or lower absorbance than the other. Another reason may be that different aged samples were used, such as a baby plant which may have a higher absorbance compared to an aging plant. Furthermore, the sun raised silverbeet plant could have had excessive exposure to the sun and was beginning to die, causing a lower absorbance. Q10. What is the single major design problem in this experiment in drawing conclusions about different concentrations of rubisco in response to different growth light conditions? (2 sentences) Rubisco is not the only protein in the plant as it makes up 80% of the total protein in plant leaves. This means that there is 20% of other existing proteins that may have contributed to the results.

The Biggest Myth About College Essay Help Exposed

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Some Of The Aspects To Write A Pro GMO Paper

Some Of The Aspects To Write A Pro GMO PaperTo get started with your research papers on GMOs and its benefits, you need to be aware of the topics in a pro-GMO paper. Now, you have a choice: You can start writing a topic by picking one of the topics provided by the primer papers.The primer papers contain topics such as: the major parties that are fighting the usage of GM products, which is of major concern in the GMO products use. You need to understand all the major parties and their supporting groups so that you are able to find out the information required for your research paper. Now, it is important to know the issues that are the major issues that are discussed in the GMO products use, as this is very essential for your research paper.The second aspect that is quite important is the description of the effects of GMOs. You need to study all the effects that are mentioned in the primer papers. This will help you make the research paper.You will need to examine all the issues, whic h are found in the primer papers, and then find out the way in which the public opinion on GMOs has evolved. In order to know the public opinion on GMOs, you need to look at the many online surveys that are done on GMOs. The online surveys will help you make the research paper. With the survey, you will be able to know how the public opinion on GMOs is evolving, and this is vital for your research paper.You should write down all the aspects in your research paper, which you think are important. Remember, you are not doing this for the purpose of getting credit, but for the purpose of developing an outline. You should also develop an outline that covers all the topics, as this will enable you to write more quickly.You can also read the GMO papers online. It will help you get the information from the public opinions, as they will also provide you with the information on the topics. There is a cost for the online studies, but there is an option to make an account that will give you all the information that you need for the research paper.Last but not the least; when you write your paper, you should make sure that you include all the aspects, which are important to the issue. If you are interested in the research papers, you should not do it all by yourself. This is so that you will not do it without having any help.These are some of the aspects that you need to be aware of if you want to write a pro-GMO paper. You need to be prepared to face all the challenges and hurdles, as this will help you write the best research paper. Make sure that you check out the entire process before you go ahead and make your own research paper.

Research Topics For Senior Paper

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Essay in School Shooting - Why it is Important to Know Your Facts

Essay in School Shooting - Why it is Important to Know Your FactsIf you are preparing for your coursework or preparing for a field trip, you may find yourself needing to prepare a sample essay in school shooting. This can be done by using word processing software and formatting it appropriately. It is important to make sure that the paper is based on the facts, so the essay should be factual, but concise and easy to read.There are a number of ways to prepare the essay, depending on how much information you need and what topics you want to cover. Some samples are easy to adapt, while others will require that you have some experience writing an essay before you can start. Let's take a look at a few ways to do this.Most people choose to use their local library for the topic of their essay, which is something that can be adapted to current events. Although this option is usually considered to be the easiest, it also allows the reader to easily skip over the information that they do not n eed to know about. Students can take this information and place it in a separate essay so that they can include it later on. In this way, you can still be able to add a bit of personal thought into the text, but you will have included the information that is important to you without going too far off track.Students who choose to write an essay in school shooting might choose to use a public source as well. However, many schools and organizations have rules about how to effectively submit a written document, and some require that you first contact the person who wrote the piece. This is usually best done when you are dealing with a private organization.If you are going to use the Internet, the best choice is to write your essay from a personal point of view. This way, you can incorporate personal thoughts that relate to the content of the essay into the main body of the paper, which can be useful. The essay should also provide as much information as possible in a short amount of time , since this is the easiest way to gather information.Most students prefer the look of handwritten essays, since this gives them a more personal touch, which is usually considered to be a good choice for essay writing. It is important to remember that you will need to include the information in a way that is easy to read and understand. You may need to expand on a subject that is already on the sample paper, or include some of the facts and details that the teacher would have included in the article if it were actually written in a school environment.Once you have used your writing skills to prepare your essay, it is a good idea to have someone else proofread the paper before it is submitted. If you go this route, make sure that the person reads the entire essay and makes any edits that are necessary. Doing it yourself will give you a better understanding of the material that you are writing about, but having a third party look over the paper will give you the chance to see how well you have done before you get into the hard work of proofreading the essay.Essays in school shooting can range from simple to complex. The essay that you write should reflect your personal viewpoint on the subject matter, so make sure that you do your homework and study before you begin writing. You can always get help from someone with more experience, or use an online tutor to teach you the basics of writing.